Posted
Thurs., April 6
Organization
Harvard Medical School and Massachusetts General Hospital Cancer Center
Position Description
Dr. Andrew Elia, MD, PhD, at Harvard Medical School and Massachusetts General Hospital Cancer Center is seeking a research technician. The candidate will assist with studies to help to find new targets for the treatment of tumors with specific DNA repair defects and will help to improve the efficacy of combining genotoxic cancer treatments with immunotherapy. Graduating seniors are encouraged to apply.
Responsibilities include:
- Characterizing novel proteins involved in DNA damage and immune signaling pathways using cell
biology and biochemistry techniques (CRISPR, RNAi, western blotting, immunofluorescence,
immunoprecipitation, chromatography, flow cytometry, and reporter gene assays).
- Performing standard molecular biology techniques (cloning, site-directed mutagenesis, production and purification of recombinant proteins).
- Performing tissue culture work doing plasmid transfection, retroviral transduction, and lentiviral
transduction.
- Assisting in CRISPR functional genomic screen to identify genes required for the selective survival of tumor cells with specific DNA repair or immune signaling defects
- Assisting with quantitative mass spectrometry proteomics to characterize novel post-translational modifications involved in the DNA damage response.
- Assisting with cancer gene mouse model.
- Establishing new and modify existing research techniques.
Position Requirements
- Bachelor degree in biology, biochemistry, chemistry, biotechnology or related science field
- 1-3 years experience in a molecular laboratory a plus
Time Commitment
Full-time, between May and August 2023.
Compensation
TBD
Apply
To apply, please send CV to Dr. Andrew Elia, MD, PhD.
